摘要
以小麦洛夫林10的成熟胚为外植体诱导愈伤,研究了不同培养基对愈伤诱导和愈伤状态的影响。结果表明:以MS+2,4-D3.0培养基的诱导效果最好,出愈率最高达94.03%;在MS+2,4-D1.0+KT0.1培养基上进行继代培养获得松脆型愈伤组织,以此培养基进行液体培养建立了状态良好的悬浮培养细胞。为进一步在单细胞水平探讨激发子诱导寄主产生防卫反应的生理机制提供了便利的试验体系。
Mature embryos of wheat lovrin10 were cultured to study the effect of different mediums on callus induction and the estate of the callus. The results showed that in MS+2,4-D 3.0 culture medium the highest ratio of callus induced was 94.03%, and in MS+2,4-D 1.0+KT 0.1 maintenanced medium the embryonal callus were obtained and cell suspension culture of wheat lovrin10 was established.These results provided a good system for further study on how the elicitor can induce the defense reaction of host.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2005年第4期68-70,共3页
Journal of Hebei Agricultural University
基金
教育部科学技术研究重点资助项目(03014)
植物生理学与生物化学国家重点实验室开放课题资助(PPB04006)
河北省自然科学基金资助(399172
301124
303180)
河北省教委博士基金资助项目(B2001211)
关键词
小麦
成熟胚
愈伤组织
悬浮培养
<Keyword>wheat
mature embryo
callus
suspension culture
