摘要
利用生物信息学方法在含有94709条棉花EST序列的数据库中进行微卫星标记筛选,共发现微卫星序列4396个,占整个EST数据库的4.64%。其中双碱基重复序列1282个、三碱基序列2411个,分别占在EST数据库中发现微卫星序列总数的29.27%和54.8%。根据筛选到的微卫星序列设计并合成引物25对,其中24对引物有扩增产物,17对产物条带比较清晰,对这些条带比较清晰的产物作了一系列的分析。
Approximately 94709 EST sequences were searched for identification of SSRs using the Simple Sequences Repeat Identification Tool (SSRIT). 4396 microsatellite sequences were obtained from 94709 EST sequences,about 4. 64% of the whole database a total of 1282(29. 2%) dinucleotide repeats and 2411(54. 8%) trinucleotide repeats were found in these microsatellite sequences. 25 primer pairs were designed from microsatellite. Among the 25 primer pairs, 17 can achieve clear PCR products by electrophoresis. The SSR markers reported in this study were highly polymorphic between Gossypium hirsutum and Gossypium arboreum but lower in Gossypium hirsutum. In order to evaluate transfera-bility of EST-SSRs,we also selected six species: Gossypium hirsutum, Zea mays, Lycopersicum es-cuenlum , Brassica napus , Beta vulgaris , Abutilon theophrasti, to detect the product. Among the product the primers Cril, Cri3, Cri4, Cri7, Cri9, Cril4 and Cril5 have products in all of these six species. Cril6, Cril7 have no product in Z. mays, L. escuentum and B. vulgaris. And Cri13 has no product in L. escuentum and B. vulgaris.
出处
《棉花学报》
CSCD
北大核心
2005年第4期211-216,共6页
Cotton Science
基金
国家863计划项目(2004AA241080)
