摘要
对培养基中,不同蔗糖浓度和活性炭含量对美国黄松不定芽继代培养的影响进行了研究.对不同蔗糖浓度下的黄松不定芽,在1/2GD和1/2SH培养基上的增殖系数作回归拟合,相关系数均达到0.98以上.计算得出:在1/2GD培养基中加入10.6071g.L-1蔗糖时,1/2SH培养基中加入20.5g.L-1蔗糖对黄松不定芽的增殖最有利.不定芽增殖系数都随着活性炭的增加而有所下降,但是,活性炭对于不定芽的伸长具有明显的促进作用.
The influences of different concentration of sucrose and activated charcoal on the Pinus ponderosa adventitious buds culture were studied. The correlations between adventitious buds propagation coefficient and different sucrose level were closed,the correlation coefficient were more than 0.98.The optimum sucrose concentration is 10.607 1 g·L^(-1) on 1/2GD medium,and (20.5 g·L^(-1)) on 1/2SH medium. The adventitious buds propagation coefficient decrease with the increasing of activated charcoal. But activated charcoal can significantly improve the buds growth.
出处
《福建林学院学报》
CSCD
北大核心
2005年第3期260-263,共4页
Journal of Fujian College of Forestry
基金
国家林业局"九四八"引进项目"美国黄松优良种源及快繁技术引进"(98-4-05)
国家林业局黄土高原林木培育实验室"美国黄松组织培养及快繁技术研究"课题(K02-06).
关键词
美国黄松
组织培养
不定芽
蔗糖
活性炭
Pinus ponderosa
tissue culture
adventitious buds
sucrose
activated charcoal
