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DNA探针在鸡传染性贫血流行病学调查中的应用

An Epizooedogical Survey to Chicken Infectious Anaemia in Jiangsu with Digoxigenin-labelled DNA Probe
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摘要 用地高辛标记的核酸(DNA)探针对江都、连云港、苏州、无锡等地3个市县9个养鸡场(户)随机取样,采集106份样品,分别为12日龄~60日龄的肉鸡、蛋鸡的胸腺、法氏囊和脾脏组织,抽提DNA检测CIAV感染情况。鸡传染性贫血病毒阳性鸡只22份,阳性率为20.7%,各鸡场阳性率从5%到50%不等。结果表明,在江苏省的一些地区鸡群中CIAV的感染已非常普遍,有的地区甚至十分严重,控制该病的发生已刻不容缓。 The DNAs of 106 thymus samples of broiler or layer chickens which were 12-60 day-old from 9 chicken farms in Jiang du, Lian yungang, Suzhou and Wu xi etc, were extracted. They were detected for CIAV DNA with digoxigenin-labelled CIAV DNA probe. Of these samples, 22(20.7%) were positive. The positive ratio ranged from 5%-50%. The results showed that CIAV infections was common in some chicken farms in Jiangsu, and some of them were seriously. So, It was of great urgency to control this disease.
出处 《动物医学进展》 CSCD 2005年第7期100-101,共2页 Progress In Veterinary Medicine
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