摘要
目的选择合适浓度的试剂以消除红细胞对酶免分析仪测定乙肝标志物的干扰。方法采用不同浓度的十六烷基三甲基溴化铵(CTAB)、尿素和草酸铵溶液分别加入到用小牛血清稀释的一系列含RBC且乙肝标志物为阴性的抗凝血浆中,用辉煌之星全自动酶免分析连体机对其进行HBsAg的测定。结果加入不同浓度的CTAB能很快使RBC溶血,但都使测定HBsAg出现假阳性;加入200、100g/L的尿素、10g/L的草酸铵可抑制低浓度RBC对HBsAg测定的影响;而360g/L的尿素和20g/L草酸铵能抑制高浓度RBC对HBsAg测定的影响,二者同时应用于32例各种已知模式的乙肝标志物的血液标本的测定(人为造成含各种浓度RBC干扰),只有360g/L的尿素能有效抑制干扰,乙肝标志物的检测结果与原模式相同。结论选择360g/L的尿素作为消除RBC干扰试剂,应用于辉煌之星全自动酶免分析连体机检测乙肝标志物能有效抑制RBC对测定的干扰。
Objective To search for suitable concentration of reagents to obviate the RBC interference in HBV marker assay using ELISA analysator. Methods The addition of different concentrations of Cetyltrimethyl Ammonium Bromide (CTAB),urea and ammonium oxalate into a serial of CS-diluted the HBV marker negative anticoagulated plasma containing RBC and measure HBsAg by using FAME-STAR ELISA Integrated Modular System. Results Various levels of CTAB induced hemolysis immediately but lead to occurrence of false positive HBsAg. However, administration of 200g/L, 100g/L urea or 10 g/L ammonium oxalate can counteract the effect of low density of RBC on the HBsAg detection. Moreover, 360g/L urea and 20g/L ammonium oxalate can inhibit the effect of high density of RBC to interfere with HBsAg assay respectively. When added to 32 various blood preparations with already known HBV marker modes (different level of RBC interference created factitiously), only 360g /L urea demonstrated effective inhibition on the interference with the same detection results as the prototype. Conclusion When administrated as antiturbulent reagent, 360g/L urea can effectively offset the RBC interference in using FAME-STAR ELISA Integrated Modular System in the assay of HBV marker.
出处
《实用预防医学》
CAS
2005年第4期784-786,共3页
Practical Preventive Medicine
