摘要
目的通过分析乙型肝炎(乙肝)病毒(HBV)血清学标志物模式,对规范乙肝临床报告的可行性进行探讨。方法用酶联免疫吸附试验(ELISA)对51746例临床标本常规HBV检测,对常见模式组用荧光定量聚合酶链反应(FQ—PCR)检测HBVDNA,对罕见模式组用ELISA进行复检。结果常见模式组占总阳性率的98%。罕见模式组经复检后,模式发生了显著变化,有的出现了多达三种不同模式的改变。结论对于HBV罕见模式,应综合分析,规范处理,方可发出HBV临床报告。
Objective To study the standard report form of HBV clinical detection by analyzing models of serological markers of hepatitis B virus(HBV) infection. Methods Fifty-one thousand seven hundred and forty-six clinical samples were test for HBsAg, anti-HBs, HBeAg, anti-HBe, anti-Hbc by ELISA. To investigate the HBV DNA, Fluorescence quantitative polymerase chain reaction (FQ-PCR) was performed on 626 samples. Result It was found that 98% samples with positive HBV serological markers were of common models. The result of rare models changed markedly when alternative detection was performed. Conclusions The variety of HBV serological models are caused by a lot of reasons, such as laboratory errors, quality of reagents, the limitation of the method, etc. Thus, a standard report form of HBV serological markers should be used to make clinical diagnosis more convenient.
出处
《实用预防医学》
CAS
2005年第4期834-836,共3页
Practical Preventive Medicine
关键词
乙型肝炎病毒
血清学标志物
荧光定量聚合酶链反应
Hepatitis B virus
Serological markers
Fluorescence quantitative polymerase chain reaction
