摘要
目的:观察地黄合剂对多发性硬化(MS)患者外周血(PB)及脑脊液(CSF)中T淋巴细胞亚群的影响。方法:采用流式细胞仪检测45例MS患者在采用地黄合剂治疗前后PB及CSF中的T淋巴细胞亚群,并进行神经功能障碍(EDSS)评分以及复发次数的统计。30例神经内科诊治的功能性疾病患者作为对照组。结果:MS患者PB中CD8+细胞显著低于对照组,CD4+/CD8+比值明显升高;CSF中CD3+、CD4+细胞明显高于对照组及PB,CD8+细胞显著低于对照组及PB,而CD4+/CD8+比值明显高于对照组及PB(以上P均<0.01)。地黄合剂能升高PB及CSF中CD8+细胞,降低CSF中CD4+细胞以及CSF和P8中CD4+/CD8+比值(以上P均<0.05)。在采用地黄合剂治疗后,EDSS评分降低(P<0.01)。复发次数减少(P<0.01)。结论:地黄合剂对MS患者PB和中枢神经系统中异常的细胞免疫功能具有有效的调节作用。
Objective:To observe the effect of dihuang mixtume on T lymphocyte subpopulation in peripheral blood(PB)and cerebrospinal fluid(CSF)of patients with multiple sclerosis ( MS). Methods:T lymphocyte subpopulations in PB and CSF of 45 cases with MS were detected by flow cytometry before and after treated with dihuang mixture, meanwhile expanded disability status scale (EDSS) and recurrence frequency were observed,30 cases with functional neural diseases as control group. Results: In PB,compared with control group, the counts of CD8 + decreased, CD4+ /CD8+ ration increased, In CSF, compared with control group and PB, CD3+ and CD4+ were significantly higher, while CD8+ lower and CD4+/CD8+ ratio higher, dihuang mixture could increase CD8+ in PB and CSF, decrease CD4+ in CSF and CD4+ /CD8+ ration in CSF and PB ( P < 0.05), After treated with dihuang mixture, EDSS mark and recurrence frequency decreased. Conclusion: Dihuang mixture could effctively regulate abnormal celluar immunity function in PB and central nervous system of patients with MS.
出处
《中国中医药科技》
CAS
2005年第4期207-208,i001-i002,共4页
Chinese Journal of Traditional Medical Science and Technology
基金
广东省科技计划项目No.261广东省中医药局科研项目No.100030
