摘要
目的研究新型HA梯度涂层人工股骨柄假体及其复合重组人骨形态发生蛋白-2(rhBMP-2)后的界面生物学特征。方法将15只健康成年杂交犬随机分成三组,行右侧人工股骨头置换,分别植入钛合金人工股骨柄(Ti组)、HA涂层钛合金人工股骨柄(HA组)和复合rhBMP-2的HA涂层钛合金人工股骨柄(BMP-HA组),12周后处死动物,取有植入假体的股骨上段进行X线检查和界面组织学观察,处死前肌内注射盐酸四环素行荧光标记。结果X线检查显示Ti组中有1例假体周围出现局部透亮带。HA组及BMP-HA组股骨柄假体周围可见新骨形成。光镜下新型HA梯度涂层无明显降解和碎裂,性质稳定。Ti组、HA组和BMP-HA组界面骨结合率分别为4.05%±7.66%、71.04%±9.81%和88.86%±6.56%。显示HA组和BMP-HA组界面骨结合良好,骨结合率显著高于Ti组(P<0.01),BMP-HA组界面骨结合率也显著高于HA组(P<0.01)。BMP-HA组的界面有较强的四环素荧光标记,显示界面成骨活跃。结论新型HA梯度涂层假体可引导骨组织长入涂层,与骨组织结合良好,结合率高,能增加假体的稳定性。该涂层可复合rhBMP-2发挥协同作用,明显增加涂层假体的骨整合,有望成为可供临床使用的新型涂层假体。
Objective To evaluate the biologic characteristics of a new type femoral stems either coated with gradient hydroxyapatite (HA) or additionally loaded with rhBMP-2. Methods Fifteen dogs were divided into 3 groups according to the implanted hip prosthesis. Each animal received hemiarthroplasty in the right hip with prosthesis made of pure Ti-6Al-4V femoral stem (Ti group), the new gradient HA-coated femoral stem (HA group) with 100 to 150 μm hydroxyapatite coating, and the new gradient HA-coated femoral stem loaded with rhBMP-2 of 9 mg (BMP-HA group) respectively. The dogs were sacrificed at 12 weeks after operation, and labeled with tetracycline 3 of 30 mg/kg days prior to sacrifice, and then the histological changes of the tissue surrounding the prosthesis were evaluated. The radiographic examinations were taken before and after sacrifice. Results On radiographic examination, local radiolucent line around the prosthesis was observed only in one specimen in Ti group. The new bone formation was found both in HA group and BMP-HA group more obviously than in Ti group. Under microscopy, it was noted that this new gradient HA-coating was intact and no obvious biodegradation in most section, whereas a fibrous membrane was found in Ti group. The average rates of interface osteointergration in Ti group, HA group and BMP-HA group were 4.05%±7.66%, 71.04%±9.81% and 88.86%±6.56%, respectively, those of HA and BMP-HA group were significantly higher than that of Ti group (P<0.01), moreover, the interface osseointegration rate in BMP-HA group was significantly higher than that in HA group (P<0.01). Strong tetracycline labeling around the interface of BMP-HA group was observed, which indicated active formation of new bone. Conclusion The type of implants with gradient HA coating provides good osteoconductivity resulting in the obvious improvement of its stability, interface osteointegration and bone formation, being enhanced significantly after loading with rhBMP-2. This implant loaded with rhBMP-2 is promising as a new type of prosthesis with novel clinical potentials.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2005年第7期400-403,共4页
Chinese Journal of Orthopaedics
基金
国家自然科学基金资助项目(59932050)
国家自然科学基金(50272021)
