摘要
目的探讨丹参、生脉治疗全身炎症反应综合征(SIRS)的作用及其机理。方法建立大鼠SIRS动物模型。40只Wistar大鼠随机分为4组,每组10只。对照组:生理盐水(5ml/kg)+脂多糖(LPS,1mg/kg);丹参组:复方丹参注射液(5ml/kg)+LPS(1mg/kg);生脉组:生脉注射液(5ml/kg)+LPS(1mg/kg);联合用药组:复方丹参注射液(2.5ml/kg)+生脉注射液(2.5ml/kg)+LPS(1mg/kg)。行血常规、血清TNFα和IL6水平检测,并行肝、肺及肾脏组织的病理组织学检查。结果丹参注射液及生脉注射液均能明显降低LPS腹腔注射后的血清TNFα和IL6水平,下调过高的炎症反应,并减轻肝、肺、肾等器官的损害,使SIRS大鼠48h存活率明显提高。结论本实验为丹参、生脉的抗SIRS临床应用提供了理论基础。
Objective To investigate the effect of Salvia Miltiorrhiza (SM) and Shengmai injection (SI) in treating systemic inflammatory response syndrome (SIRS) and their mechanism. Methods The animal model of SIRS was established by injecting lipopolysaccharide(LPS, 1 mg/kg)intraperitoneally. Forty Wistar rats were randomly divided into four groups: control group, SM group, SI group and combined treatment group (SM+SI group), which were treated with normal saline(5 ml/kg) plus LPS(1 mg/kg), SM(5 ml/kg)plus LPS(1 mg/kg), (SI(5 ml/kg))plus LPS(1 mg/kg), SM(2.5 ml/kg) plus SI(2.5 ml/kg) and LPS(1 mg/kg) respectively. Six rats of each group were sacrificed for sample collection of blood, liver, lung and kidney 8 hours after LPS injection. Blood routine, serum TNF-α and IL-6 were measured. Specimen of organs were fixed in formalin and sent for routine pathological examination. The survival of other 4 rats of each group were observed untill 48 hours after LPS injection. SPSS 10.0 was used in statistical analysis. Results Two rats in control group died 13 hours and 22 hours after LPS injection respectively, the remaining 2 rats in this group and the rats in other 3 groups survived 48 hours after LPS injection. The white blood cell count of control group was significantly higher than that of other groups. The serum TNF-α and IL-6 of control group were significantly more than those of other groups. Pathological damages were found in all groups, and the most severe ones were in control group. SM and SI could decrease the level of serum TNF-α and IL-6 in the process of LPS-stimulated SIRS, down-regulate the severe inflammatory response, attenuate organ damages of the liver, lung and kidney, and increase forty-eihgt-hour survival rate obviously. Conclusion The experiment provides a theoretical base for clinical use of SM and SI in treatment of SIRS.
出处
《中国普外基础与临床杂志》
CAS
2005年第4期395-398,共4页
Chinese Journal of Bases and Clinics In General Surgery
