摘要
目的:探讨p15、p16、Rb、p27、p53及PCNA基因蛋白在不同分期、不同部位和不同分级的喉鳞状细胞癌(LSCC)中的表达和临床意义。方法:用免疫组织化学染色SP法对65例LSCC进行染色,30例正常黏膜为对照组,检测p15、p16、Rb、p27、p53及PCNA基因蛋白的表达,并进行统计学分析。结果:6种基因蛋白的表达在正常黏膜和LSCC中的差异有统计学意义(均P<0.01)。且p15和p27表达不仅和LSCC生长部位相关,而且和临床分期、分化程度相关。p16蛋白表达与LSCC的病理分级呈负相关,PCNA蛋白的表达与LSCC的病理分级呈正相关,两者的表达均与临床分期和肿瘤生长部位无关。而LSCC生长越晚期,Rb、p53蛋白的表达,前者呈下降,后者呈上升状态,且p53蛋白的表达和恶性程度呈正相关。结论:p15和p27基因表达的缺失是LSCC发生发展的重要过程,对两者的检测在LSCC的治疗和预后方面有一定意义。p16和PCNA反映肿瘤细胞的增殖状态,与肿瘤的发生有关,与肿瘤的发展过程无关。而Rb、p53基因表达多与肿瘤的发展过程相关。
Objective:To investigate the expression of p15,p16,Rb,p27,p53 and PCNA gene proteins in different clinical stage,different position and different histological types of laryngeal carcinomas and their significance.Method:p15,p16,Rb,p27,p53 and PCNA gene proteins were examined with immunohistochemical SP method in the paraffin-embedded specimens of 65 cases with LSCC and 30 cases of normal or proliferative mucosas of larynx as a contrast.The result was analyzed by chi-square test.Result:The expression of the six gene proteins in LSCC and in laryngeal with normal or proliferative mucosas was different,the difference was significant(P<(0.01));the expression of p15 and p27 was significantly related with the position/clinical stage/histological types of LSCC(P<(0.01));the expression of p16 was significantly negative but the PCNA was positive correlated with the histological types of LSCC(P<0.01).Two of them did not correlate with the position or clinical stage of LSCC(P>(0.05)).There was trend of the decreasing Rb expression or increasing p53 expression with increasing clinical stages of LSCC, and the expression of p53 was also positive correlated with the histological types of LSCC.Conclusion:p15 and p27 play an important role in the development and progression of LSCC, and can be used as markers for assessment the treatment and prognosis of LSCC.The expression of p16and PCNA can reflect the proliferation activity in the tumor cells,and is correlated with the development of tumor, but it does not correlate with the progression of tumor.The expression of Rb and p53 were more correlated with the progression of tumor.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2005年第14期648-651,共4页
Journal of Clinical Otorhinolaryngology
关键词
喉肿瘤
癌
鳞状细胞
基因表达
Laryngeal neoplasms
Carcinomas,squamous cell
Gene expression
