摘要
目的比较肝癌和正常肝组织的缺血再灌注损伤。方法兔肝脏注射VX2肿瘤组织混悬液建立肝脏肿瘤,和缺血再灌注模型。测定再灌注各时点组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量;用HE和TUNEL染色观察和比较两种组织的细胞凋亡情况。结果缺血再灌注后癌组织中的SOD浓度下降显著,于再灌注1h即达最低水平(64.59±4.97NU/mgprot),其后逐渐恢复,但至再灌注7d仍低于再灌注前(121.12±6.88NU/mgprot)。与正常肝组织比,癌组织缺血再灌注后MDA的浓度下降,变化与SOD相似;凋亡细胞数量明显增加,至1d时达最高水平;至再灌注7d时阳性细胞仍多于缺血再灌注前,其凋亡细胞数量多于正常肝组织。结论癌组织对缺血再灌注的影响和损伤较正常肝组织更为敏感。
ObjectiveTo compare the injury to hepatocarcinoma and normal liver tissues following ischemia and reperfusion. MethodsThe hepatocarcinoma(HC) models were established by injectin of VX2 tumor (tissue) suspension fluid into the left-middle lobe of liver of rabbits. After the models were established, the models left hepatic vessels were occluded for 60 minus, then followed by various intervals of reperfusion, and the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured, and apoptotic (changes) in the hepatocarcinoma and normal hepatic tissues were observed by means of HE staining and TUNEL method. ResultsThe concentration of SOD decreased significantly in hepatocarcinoma tissue and reached the lowest point at 1h after ischemia and reperfusion (64.594.97NU/mgprot), then gradually rebounded, but, at 7d after reperfusion, it still retained at a lower level(121.126.88NU/mgprot) than that before reperfusion. In contrast with normal liver tissues,the concentration of MDA decreased in the hepatocarcinoma tissues following ischemia and reperfusion, the changs of MDA were similar to SOD. The apoptotic cells in hepatocarcinoma tissues increased to the highest point at 1 day following reperfusion, but 7d after reperfusion, the number of positive cells were more than before reperfusion, and the apoptotic rate was higher in (hepatocarcinoma) tissues compared with the normal liver tissues. ConclusionsIn comparison with normal liver tissue, hepatocarcinoma tissue is more suscepible to the injury of ischemia and reperfusion.
出处
《中国普通外科杂志》
CAS
CSCD
2005年第7期497-500,共4页
China Journal of General Surgery
基金
陕西省科技攻关资助项目(2004KB-G10(2))。
关键词
肝肿瘤/血液供给
肝/血液供给
再灌注损伤
<Keyword>Liver Neoplasms/blood suppl
Liver/blood suppl
Reperfusion Injury
