摘要
An important, but often limiting step in marker-assisted breeding is the efficient isolation of plant DNA for polymerasechain reaction (PCR) amplification. A simple method using an alkali treatment to extract wheat DNA for marker-assistedselection (MAS) in wheat breeding programs was compared to a commercial kit and cetyltrimethylammonium bromide(CTAB) extraction. DNA concentration from the alkali extraction was higher than the other two methods but purity waslower than CTAB extraction. The alkali extraction method was used on breeding lines to determine its usefulness. Thealkali-extracted DNA samples were suitable for several PCR-based procedures, including random amplified polymorphicDNA (RAPD), microsatellite (simple sequence repeat, i.e., SSR) and sequence characterized amplified region (SCAR)analyses.
An important, but often limiting step in marker-assisted breeding is the efficient isolation of plant DNA for polymerasechain reaction (PCR) amplification. A simple method using an alkali treatment to extract wheat DNA for marker-assistedselection (MAS) in wheat breeding programs was compared to a commercial kit and cetyltrimethylammonium bromide(CTAB) extraction. DNA concentration from the alkali extraction was higher than the other two methods but purity waslower than CTAB extraction. The alkali extraction method was used on breeding lines to determine its usefulness. Thealkali-extracted DNA samples were suitable for several PCR-based procedures, including random amplified polymorphicDNA (RAPD), microsatellite (simple sequence repeat, i.e., SSR) and sequence characterized amplified region (SCAR)analyses.
