摘要
目的:探讨乙型肝炎患者血清中乙肝病毒DNA、谷丙转氨酶(ALT)、总胆汁酸(TBA)与一氧化氮(NitricOxide,NO)和一氧化氮合酶(Nitricoxidesynthase,NOS)水平的相关性,以求证实乙肝病毒在体内复制,损伤肝细胞,引起慢性纤维化和肝硬变乃至发生肝癌的疾病进程中,NO和NOS所起的作用及意义。方法:选取42例慢性持续性肝炎(CPH)患者,30例慢性活动性肝炎(CAH)患者,31例乙型肝炎肝硬化(HC)患者,19例乙肝病毒相关性肝癌(HCC)患者;30例健康体检者作为对照。分别运用荧光定量PCR技术和化学分析法对上述病例的血样同时进行乙肝病毒DNA含量、ALT、TBA、NO和NOS水平的检测。结果:对照组、HBV-DNA阴性组与HBV-DNA低浓度水平组之间的NO、NOS的水平无显著性差异(P>0.05)。HBV-DNA低浓度水平组与中、高浓度组之间的NO、NOS水平有显著性差异(P<0.05)。慢性活动性肝炎组,乙型肝炎肝硬化组,乙肝病毒相关性肝癌组与对照组间HBV-DNA、ALT、TBA、NO、NOS含量之间均存在显著性差异(P<0.05)。慢性持续性肝炎组的NO水平则低于正常对照组(P<0.05)。HBV-DNA的含量(取对数值)与NO水平呈正相关(r=0.687)。结论:NO在乙肝病毒感染所导致的一系列连锁疾病的多个阶段扮演着重要角色,在不同类型的乙肝患者中NO水平的高低与病毒复制强弱和肝细胞损伤程度有关。在检测ALT,TBA的同时,连续监测乙肝患者血清中HBV-DNA含量及NO、NOS的水平对了解乙肝患者病情的演变和预后以及治疗起着重要作用。
Objective:By detecting the relativity between HBV-DNA, ALT, TBA , and NO,NOS levels in serum of chronic Hepatitis B virus infected individuals, to confirm their meanings inthecourse of HBV replication, damaging liver cells, producing fibrosis and liver cirrhosisoccurrenceof hepotocellular carcinoma. Methods:42 patients with CPH, 30 patients with CAH, 31 patientswith hepatic cirrhosis and 19 patients with hepotocellular carcinoma; 30 individuals without liverdiseases were studied. The HBV-DNA, ALT, TBA, NO, NOS in serum were detected by FQ-PCR and chemical analysis respectively at the same time.Results:Therewas no significant differencein the levels of NO, NOS among the negative control group, the HBV-DNA negative group and thelow contains group(P > 0.05). There was significant difference in the levels of NO, NOS amongthe low contains group, the medium contains and the high contains group(P < 0.05). The levelsof HBV-DNA, ALT, TBA, NO and NOS in CAH, hepatic cirrhosis and hepotocellular carcinomawere all difference with the controlling group significantly (P < 0.05 ). The content of NO in CPHwas lower than that in negative control(P < 0.05). The content of HBV-DNA (logarithms value)was positive relative to the level of NO(r = 0.687). Conclusion:NO played the important role inthe some stage of HBV causing a series of infection. The variety of the levels of NO in kinds ofHBV infections was related to the degree of virus replication and infection. Analyzing the contentsof ALT and TBA, it was important to detect the contents of HBV-DNA, NO, NOS in serum con-tinuously for the changing , consequence and therapy of HBV patients.
出处
《江汉大学学报（自然科学版）》
2005年第2期31-34,共4页
Journal of Jianghan University：Natural Science Edition
