摘要
目的探讨益髓生血颗粒治疗β珠蛋白生成障碍性贫血的作用机制。方法观察20例患儿治疗前后血红蛋白(Hb)、红细胞(RBC)、网织红细胞计数(Ret)和胎儿血红蛋白(HbF)的改变;选取3例有效病例,通过mRNA差异显示技术(DDRTPCR)研究益髓生血颗粒对β珠蛋白生成障碍性贫血患儿骨髓有核细胞的基因表达差异。结果与治疗前比较治疗后患儿各项血液学参数均有提高(P<0.05或P<0.01);患儿骨髓有核细胞的铁蛋白基因表达明显降低。结论益髓生血颗粒可以改善患儿的临床症状,明显降低患儿铁蛋白基因的表达,有效缓解患儿体内铁的蓄积,这可能是益髓生血颗粒治疗本病的分子机制之一。
ObjectiveTo investigate the mechanism of Yisui Shengxue granule (YSG) in children with β-thalassemia. MethodsThe changes of hemoglobin (Hb), red blood cells (RBC), reticulocyte (Ret) and fetal hemoglobin (HbF) of 20 children with β-thalassemia were measured before and after treatment. The effect of YSG on gene expression in myelo-karyocytes in 3 selected children treated effectively by YSG was determined by using DDRT-PCR. ResultsThe hematologic parameters were significantly enhanced and the ferritin gene expression declined after treatment respectively, as compared with those before treatment, the difference were significant (P<0,01 or P<0.05). ConclusionYSG could improve the clinical symptoms of children with β-thalassemia. One of its therapeutic mechanisms may be its action in decreasing ferritin gene expression so as to effectively relieve the accumulation of iron in body.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2005年第7期591-594,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30171199)
国家自然科学基金重大研究计划(No.90409003)
