摘要
目的 观察大鼠中度低温体外循环(CPB)后认知功能变化。方法 雄性SD大鼠随机分为CPB组(n =6 )、假CPB组(Sham组,n =4 )及单纯麻醉组(n =4 )。所有动物在咪唑安定、芬太尼麻醉后经口插管控制呼吸。CPB组和假手术对照组置入颈静脉流出管和尾动脉输入管,肝素抗凝(5 0 0U/kg)。CPB组采用中度低温CPB(2 6℃～2 8℃) ,经尾动脉灌注、颈静脉右心房-腔静脉引流,灌流量16 0ml·kg-1·min-1,总转流时间2h。Sham组除不经历CPB外,其余操作同CPB组相同,单纯麻醉组除麻醉机械通气外不经历任何手术操作。术后3d采用旷场分析(Openfieldtest )观察5min内动物的跨格次数、中央格停留时间、站立次数和排便粒数,分析术后大鼠兴奋性、紧张程度及空间认知功能变化。结果 术后3d ,CPB组大鼠兴奋性、紧张程度及空间认知功能与Sham组和单纯麻醉组相比显著降低。结论 中度低温CPB可引起大鼠认知功能损害。
Objective To observe cognitive function changes after moderate hypothermic cardiopulmonary bypass in rats.Methods Male SD rats were randomly divided into three groups: CPB group(n=6), sham-CPB group (Sham group, n=4) and anesthesia group(n=4). All animals were anesthetized (Midazolam, fentanyl, IP), orally intubated, and mechanically ventilated. In CPB group and Sham group, jugular venous outflow catheter, tail arterial inflow catheter and femoral arterial catheter were inserted, and systemic anticoagulation was achieved with heparin (500 U/kg). Animals selected for CPB underwent 2 h moderate hypothermic (26 ℃～28 ℃) CPB with a hemodilution at a flow of 160 ml·kg -1 ·min -1 , whereas animals of sham group without CPB intervention during this interval, and anesthesia group only underwent anesthesia and mechanically ventilation without any other interventions. At 3 day after CPB, the cognitive function was determined using 'Open field test' method. Results At 3 day after CPB,excitability, nervousness degree and spatial probe function were significantly decreased in CPB group compared with those in sham and Anesthesia groups.Conculsion Conclusion Moderate hypothermic cardiopulmonary bypass can induce cognitive decline.
出处
《国外医学（麻醉学与复苏分册）》
2005年第1期9-11,共3页
Foreign Medical Sciences(Anesthesilolgy and Resuscitation)
