摘要
目的:研究西红花苷对培养的牛血管内皮细胞凋亡的影响。方法:采用3β、5α、6β三羟胆固(烷)醇(Triol)诱导内皮细胞凋亡的模型,用比色法测定丙二醛含量,光学显微镜、电子显微镜检测形态和超微结构的变化,DNA电泳检测DNAladder及流式细胞仪分析法检测凋亡率,采用逆转录聚合酶链式反应(RTPCR)法检测BaxmRNA表达的变化。结果:Triol处理后,培养液中MDA含量增加,为1.761nmol·L-1(P<0.01),细胞收缩,核浓缩,深染,线粒体肿胀空化,出现凋亡小体,出现凋亡典型的“DNAladder”,和亚二倍体峰,凋亡率为30.62%,BaxmRNA表达量增加;西红花苷(10-7,10-6mol·L-1)+Triol组,MDA含量减少,内皮细胞形态结构的完整,凋亡率分别为24.4%,6.3%,BaxmRNA表达量降低。结论:西红花苷可能是抗脂质过氧化并通过调节BaxmRNA表达,减少细胞异常凋亡。
AIM: To investigate effects of Crocin on apoptosis of vascular endothelial cells injured by triol. METHODS: Apoptosis model induced by triol(cholestane-3β,5α,6β-triol)was adopted. Malondialdehyde (MDA) content was measured by colorimetric assay. The morphology and ultrastructure were detected by light microscope and electron microscope. DNA ladder was analyzed by DNA electrophoresis. The apoptosis rate was assessed with the flow cytometer assay. Bax mRNA expression of endothelial cells was measured by RT-PCR analysis. RESULTS: In Triol group, MDA content was 1.761 nmol·L -1 (P< 0.01). BAECs was shrinkage. The nucleus concentrated and dye deeply, mitochondria swelling and cavitations, mitochondrial cristae fragmentation, formation of apoptotic bodies, the DNA ladder in agarose gel and hypodiploid cusp was observed. Apoptosis rate was 30.62%. Bax mRNA expression increased. In the Crocin +Triol group, MDA content decreased, majority of cells grown in spreading out like that in control and cholesterol group. Apoptosis rate of Crocin (10 -7 or 10 -6 mol·L -1)+Triol group were 24.4%, 6.3%, respectively. Bax mRNA expression of endothelial cells in the crocin (10 -6 mol·L -1) +Triol group decreased. CONCLUSION: Crocin maybe abnormally decrease apoptosis by anti-lipid peroxidation and adjust Bax mRNA expression.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第6期627-632,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
