摘要
目的腭裂手术创口是一类不易愈合的创口,为促进腭裂术后创口愈合速度与质量,减少术后复裂率,在腭裂动物模型上对脱已酰壳聚糖的作用进行药效学研究。方法选20只Wistar大鼠随机分4组,以两种不同术式的实验组与相应的对照组进行实验。术后1、3、5、7、10天分别进行临床观察和组织病理学观察。结果实验组1,术后1天创面有缺血,3和5天粘膜颜色基本正常,7和10天拆线后的创口均为一期愈合。对照组1,术后1天创面有缺血,3和5天创缘糜烂,7天减轻,10天拆线创口二期愈合。实验组2与其对照组均有显著差异。组织病理,实验组1和组2重建覆盖上皮较对照组出现的早,实验组2缺损断端有新形成的骨组织连接,新骨周边可见成骨细胞,对照组1,有成纤维连接和炎细胞浸润。对照组2,缺损断处有新形成的软骨细胞,有炎细胞浸润。结论脱已酰壳聚糖对腭裂术后的愈合有较明显的作用,有深入研究和临床应用的价值。
Objective To study the medical effect of chitosan on improving postoperative treatment and quality of cure of cleft palate and on reducing recurring rate in animal model.Methods Twenty wistar mice were randomly devided into four groups; the experiment 1 and control 1, who were consist of complete cleft palate. The experiment 2 and control 2, who were consist of incomplete cleft palate. All of the animals were observed clinically and histologically on day 1, 3, 5, 7, and 10.Results Clinical observations: on day 1, the woumd surfaces showed ischemia; on day 3 and 5 the mucous color was normal; on day 7 and 10 the first stage cure occurred. There was significant difference betweet the experiment 1 and the experiment 2. Histological observations: the reconstruction of epithialum occurred earlier in experimental groups; the wound borders were linked by new bones with osteoblasts around. But in control 1,the wound borders were linked by fiber with inflammation cells infiltrating. In the control 2,the chondrocytes and inflammation cells were found nearby the wound borders.Conclusion There was difference in improving postoperative cure of cleft palate with the chitosan treatment. It is necessary to further conduct more research in clinical applications.
出处
《现代口腔医学杂志》
CAS
CSCD
北大核心
2005年第4期405-406,共2页
Journal of Modern Stomatology
