摘要
根据GenBank上登录的鸡IL2基因序列设计合成了1对特异性引物,以ConA诱导的鸡脾淋巴细胞提取的总RNA为模板进行RTPCR。结果显示,扩增出一450bp的目的片段,将该基因片段克隆到pBluescriptⅡKS(+)载体上,经酶切鉴定及序列测定证实为鸡IL2基因,与GenBank上已知的鸡IL2基因相比,其在编码氨基酸的第49位有一个氨基酸的缺失,序列分析表明,该基因是目前发现的在该位置唯一有氨基酸缺失的鸡IL2基因。另外,它与Broiler、SC和Xiaoshan鸡在编码的氨基酸上完全一致,具有较近的亲缘关系,而与Kestrel来航鸡、来航SPF鸡、Obese、Silky、Chenren和Xianju鸡在编码的氨基酸上有1～4个氨基酸的差异。
The chicken interleukin-2 gene, a cDNA fragment about 450bp in length, was amplified from ConA-stimulated chicken spleen cells by RT-PCR using a pair of primers which were designed and synthesized according to the ChIL-2 gene sequence in GenBank published by Sundick. The gene was inser- ted into pBluescriptⅡKS(+) and identified by endonuclease and DNA sequencing. The result of sequence analysisshowed that the gene encoding a protein of 142 amino acids residues. An amino acid absence was found at the region of 49 compared with all other published chicken IL-2 genes. The cloned gene was the only chicken IL-2 gene that an amino acid is absent at the region. The other amino acids were consistent with that of Broiler, SC and Xiaoshan chickens and there are close relationships among them, but there is 1-4 amino acids difference between the deduced amino acids sequence from the cloned gene and that from Kestrel Leghorn, Leghorn SPF, Obese, Silky, Chenren and Xianju chickens.
出处
《中国兽医科技》
CAS
CSCD
北大核心
2005年第7期555-560,共6页
Chinese Journal of Veterinary Science and Technology
