摘要
目的探讨原发性肢体淋巴水肿发生、发展过程中部分可能相关基因的表达和功能。方法选择单侧下肢原发性淋巴水肿患者,分别于患肢与健肢对称部位取皮肤和皮下组织样本,抽提RNA,制备探针;选用H40s表达谱芯片进行杂交和洗涤;ScanArray4000扫描芯片,GenePixPro3.0软件分析扫描结果;最后在Medline数据库中查询阳性基因的功能并进行生物信息学分析。结果在4096种基因中,原发性淋巴水肿患肢与健肢存在基因表达差异;所检测的4对临床标本共同的差异表达基因为27条(0.66%);该27条基因与原发性肢体淋巴水肿的发生机制可能存在相关性。结论基因表达谱芯片能够筛选出在原发性肢体淋巴水肿的发生、发展过程中存在的多个基因表达调控的改变。
ObjectiveTo study some possible related genes in the appearance and development of primary lymphedema in limbs and discuss their function.MethodsYoung female patients with primary lymphedema in one limb were chosen to study. A piece of skin with subcutaneous tissues were incised from the symmetric sites of the affected and normal limb respectively. Then RNA were isolated and prepared as cDNA probes. After hybridization and washing, scanning was carried out by ScanArray 4000 of General Scanning Corporation and analysis by GenePix Pro 3.0. Finally, the functions of the positive genes were enrolled into the Medline data base and bioinformatical analysis were proceeded.ResultsAmong the 4 096 target genes, there were 27((0.66%)) genes whose expression levels differed between the affected and normal limbs. These 27 genes were possibly related to the molecular mechanism of primary lymphedema in limbs.ConclusionDNA microarray technology is an effective technique in screening for differentially expressed genes between the affected and normal limbs with primary lymphedema.
出处
《上海第二医科大学学报》
CSCD
北大核心
2005年第7期683-686,共4页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市教委高校科技发展基金(02BK03)资助项目.
关键词
原发性淋巴水肿
基因表达
发病机制
基因芯片
<Keyword>primary lymphedema
gene express
molecular mechanism
DNA microarray
