摘要
目的研究贵州小型香猪来源的组织工程皮肤自体及同种异体移植后基底膜形成情况,为复合壳多糖组织工程皮肤临床应用提供实验依据。方法按我科已建立的方法构建猪的组织工程皮肤,然后进行自体及同种异体移植实验,观察移植的猪组织工程皮肤的存活及创面愈合情况,并于移植后1、4、8周取标本,行组织学检查和免疫组化染色,观察移植后猪组织工程皮肤基底膜形成情况。结果猪组织工程皮肤自体及同种异体移植后均存活,创面达到理想的愈合效果,组织学检查显示表皮明显增厚,表皮突明显,真皮胶原束显著增粗变密,新生血管网丰富。PAS染色基底膜带呈完整连续阳性。免疫组化染色显示:表、真皮交界处层粘连蛋白抗体免疫组化染色阳性。结论我们构建的猪组织工程皮肤自体及同种异体移植后,可获得理想的创面愈合效果,组织工程皮肤基底膜形成完整,与正常皮肤相似。本实验为复合壳多糖组织工程皮肤临床应用提供了有力的实验依据。
Objective To provide experimental evidence for clinical application of the composite chitosan tissue engineered skin, a swine tissue engineered skin was fabricated to study the reconstruction of the blood vessels after autograft and allograft.Methods Serially passaged cells were used to fabricate the composite chitosan tissue engineered skin.Then the tissue engineered skin were autografted and allografted onto full thickness excised wounds of two swines after organotypic culture in vitro.Its viability and wounds healing status were observed.Biopsy of the grafts taken at 1st,4th and 8th week respectively were examined by histologically and immunohistochemically to investigate reconstruction of the basement membrane of the tissue engineered skin.Results The tissue engineered skin were living after autograft and allograft with the surrounding normal skin of the swine.The wounds healed well.The tissue engineered skin transplanted onto the swine showed immunohistochemically that laminin was expressed.ConclusionWounds maybe healed well after autograft and allograft.The basement membrane of tissue engineered skin is well.This animal study provides experimental evidence for clinical application of the composite chitosan tissue engineered skin.
出处
《实用医药杂志》
2005年第7期615-618,共4页
Practical Journal of Medicine & Pharmacy
基金
国家自然科学基金资助课题(NO309870238)
关键词
组织工程皮肤
自体移植
异体移植
基底膜
Tissue engineered skin Autograft Allograft Basement membrane
