摘要
目的:建立了齐多夫定的有关物质[有关物质B(3’-氯-3’-脱氧胸苷)、有关物质C(胸苷)]的HPLC检测方法。方法:采用Lichrospher-C18色谱柱(250 mm×4.6 mm,5μm);流动相A为水,流动相B为甲醇,采用梯度洗脱,10 min后流动相 A在40 min内从70%降至30%,流速1.0 mL·min-1,检测波长265 nm。结果:有关物质B、C和齐多夫定的最低检测限量分别为4.4,2.6,4.0 ng,齐多夫定与各杂质分离良好。结论:本法简便快速,灵敏度高,准确可靠。
Objective:To establish an HPLC method for detection of the zidovudine's related substances(related substance B:3'-chloro-3'-deoxynucleoside,related substance C:thymine).Methods:The analytical column was Lichrospher -C18 column(250 mm×4.6 mm,5μm). Using gradient elution method,the mobile phase A was water and the mobile phase B was methanol(ten minutes later,the mobile phase A decreased from 70% to 30% in forty minutes). The flow rate was 1.0 mL·min-1. The detection wavelength was 265 nm. Results:The detection limits for related substances B,C and zidovudine were 4. 4,2.6 and 4.0 ng respectively. Good resolution for zidovudine and related substances was obtained. Conclusions:The method is simple,rapid,sensitive and accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第7期856-858,共3页
Chinese Journal of Pharmaceutical Analysis
