摘要
目的:观察基质金属蛋白酶9(MMP9)及其mRNA在胃癌间质中的表达情况及其与肿瘤浸润、转移及间质血管新生的关系。方法:利用74例胃癌标本,经连续切片免疫组织化学染色、原位杂交及形态定量分析法,进行MMP9、MMP9mRNA、CD34及αSMA的检测,并结合临床病理参数进行综合分析。结果:MMP9及其mRNA的表达均与癌组织的浸润深度、分化程度、淋巴结转移有关;间质微血管密度(MVD)与癌组织的浸润深度、淋巴结转移有关;24例MMP9表达于血管内皮细胞和周细胞:4例间质毛细血管内皮细胞MMP9mRNA阳性,其中3例血管壁周细胞MMP9mRNA阳性。结论:胃癌细胞自身具有产生与分泌MMP9的能力,MMP9对肿瘤的侵润、转移及间质血管新生具有促进作用。
Objective To investigate the expressions of MMP-9 and MMP mRNA in gastric carcinoma interstitium and the relationships between the expressions and the invasion,metastasis and angiogenesis of the neoplasm.Methods MMP-9,MMP-9 mRNA, CD34, α-SMA in 74 cases of gastric carcinoma were examined by immunohistochemistry, in situ hybridization and morphometric analysis of secquential slides. The relationships between the expressions of MMP, MMP mRNA and the clinical parameters were analysed. Results The expressions of MMP-9 and MMP mRNA were closely related to the invasion, differentiation and lymph node status of the gastric carcinoma. The microvessel density (MVD) had great correlation with the invasion and lymph node status of the carcinoma. Endothelia and pericytes were MMP-9 positive in 24 cases.Endothelia were MMP-9 mRNA positive in 4 cases among which pericytes of 3 cases were MMP-9 mRNA positive.Conclusion Gastric carcinoma cells have the ability to produce and secret MMP-9 which can promote the invasion,metastasis and angiogenesis of the neoplasm.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2005年第4期584-587,共4页
Journal of Jilin University：Medicine Edition
基金
教育部博士学科点专项科研基金(20010183001)
关键词
胃肿瘤
明胶酶B
血管新生
肿瘤侵润
肿瘤转移
stomach neoplasm
gelatinase B
angiogenesis
neoplasm invasiveness
neoplasm metastasis
