摘要
目的探讨检测外周血T淋巴细胞核仁组成区(nucleoar organizer regions,NORs)嗜银蛋白(Ag-NoRs)在肿瘤诊断中的价值。方法分别取1100位健康查体者(健康组)、336例经病理确诊的肿瘤患者(肿瘤组)及418例非肿瘤患者(良性病组)的静脉血0.5mL,经培养、制片、银染、图象分析,以T淋巴细胞核仁银染面积与细胞核面积比值作为Ag-NoRs的检测指标。结果健康组、良性病组及恶性肿瘤组外周血T淋巴细胞Ag-NORs检测结果分别为(7.23±0.45)%、(7.03±0.54)%和(4.03±0.25)%。经统计学分析,健康组、良性病组分别与恶性肿瘤组比较差异有统计学意义,t=32.34,P<0.05;t=29.13,P<0.05;但良性病组与健康组之间差异无统计学意义,t=7.32,P>0.05。结论外周血T淋巴细胞Ag-NoRs含量测定对肿瘤的诊断具有重要意义。
OBJECTIVE: To evaluate the analysis of nucleolar organizer region associated argfrofil proteins (Ag-NORs) in peripheral blood T lymphatic cell for the diagnosis of malignant tumor. METHEDS: Perpheral blood (0.5 mL) from 1 100 normal volunteers (healthy team) and 336 patients with malignant tumor proved by pathology (malignant tumor team) and 418 patients with benign disease (benign disease team) was cultured, smeared, sliver-dyed and analized. I.S% (integral areas/nucleus integral areas) was as the index of Ag-NORs. RESULTS: The mean of I.S% was (7.23±0.45)% in healthy team, 4.03± 0.25 in malignant tumor team and (7.03±0.54)% in benign disease team. A significant difference was found (P<0.05) between the malignant tumor team and healthy team or benign disease team, but there was no statistically significant difference ( P> 0.05) between the healthy team and benign disease team. CONCLUSION: The analysis of Ag-NORs in peripheral blood T lymphatic cell has significant value in the diagnosis of malignant tumor.
出处
《肿瘤防治杂志》
2005年第14期1087-1089,共3页
China Journal of Cancer Prevention and Treatment
关键词
肿瘤
诊断
淋巴细胞
核仁组成区
neoplasms
diagnosis
lymphocytes
nucleolus organizer region
