摘要
目的研究菝葜的抗炎活性成分及其抗炎作用。方法采用反复硅胶柱层析法对菝葜乙酸乙酯部位的化学成分进行分离纯化,分别采用红外、质谱、磁共振(包括1H-NMR、13C-NMR及2D-NMR)方法鉴定所得化合物的结构。并通过大鼠灌胃给药,考察所得化合物对大鼠棉球肉芽肿的影响。结果共分离鉴定了2种化合物,即白藜芦醇(化合物Ⅰ)和氧化白藜芦醇(化合物Ⅱ)。化合物Ⅰ0.2mg·kg-1·d-1对大鼠棉球肉芽肿有显著的抑制作用(P<0.05)。结论白藜芦醇是菝葜的抗炎活性成分之一。
Objective To study the active anti-inflammatory components of Smilax China and their anti-inflammatory effects in rats. Methods The chemical components of the ethyl acetate portion of Similax China were separated and purified repeatedly with silica gel column chromatography. The structures of the chemical compounds Ⅰ and Ⅱ thus obtained were then authenticated with infrared analysis, mass spectrometry and nuclear magnetic resonance (including ~1H-NMR,~13C-NMR and 2D-NMR),separately. The anti-inflammatory activity of the compounds Ⅰ and Ⅱ was assayed by observing the effect of the compounds on the cotton ball-induced granuloma in rats after these compounds had been administered by gastrogavage. Results The compoundsⅠandⅡ isolated were identified as reverstroal(Ⅰ) and oxyreverstroal(Ⅱ). Reverstroal (Ⅰ) in a dose of 0.2 mg·kg^-1·d^-1 was shown to inhibit the cotton ball-induced granuloma in rats(P<0.05). Conclusion Reverstroal (Ⅰ) was found to be one of the anti-inflammatory components of Smilax China.
出处
《医药导报》
CAS
2005年第8期670-672,共3页
Herald of Medicine
关键词
白藜芦醇
氧化白藜芦醇
菝葜
化学成分
抗炎作用
Reverstroal
Oxyreverstroal
Smilax China
Chemical components
Anti-inflammatory activity
