摘要
为确定我国青蟹的分类地位及其资源的合理利用与保护提供理论依据,对中国和越南青蟹的线粒体16SrRNA基因片段序列进行了测定,并同Genbank中其他青蟹的序列进行了比较,分析研究了青蟹种内和种间的遗传差异及其分类地位。结果显示:中、越青蟹个体间序列差异极小,与Genbank中Scyllaparamamosain同源片段序列的相似性达到99%以上,而与其他3种青蟹的差异达3.91%～8.41%。所得序列与S.paramamosain,S.serrata,S.olivacea及S.tranquebarica的遗传距离分别为0.002,0.075,0.087,0.042,种间遗传距离远大于种内距离。序列特征、遗传距离和系统进化分析结果都表明本文研究的中国和越南青蟹均为S.paramamosain。
This paper deals with the sequence analysis of mitochondrial 16S rRNA gene fragment of mud crabs collected from China and Vietnam. The result showed that there was least sequence variations among the samples. The similarity between these sequences and sequence Scylla paramamosain was in Genbank over 99%, But the diffence with the other three species were 3.91%～8.41%. Based on Kimura's 2-parameter model, the distances between the sequences with S. paramamosain, S. serrata, S. olivacea, and S. tranquebarica were 0.002, 0.075, 0.087, and 0.042 respectively which the interspecific genetic distance was much larger than intraspecific distance from composition, genetic distance and phylogenetic analysis suggested that the samples from China and Vietnam should be S. paramamosain.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第4期554-558,共5页
Periodical of Ocean University of China
基金
国家自然科学基金项目(30471329)资助
关键词
青蟹
16S
RRNA基因
序列
分类
mud crabs
16S rRNA gene
sequence
species identification
