摘要
目的研究L-arg对大鼠皮脂腺增生作用机制的研究。方法雌、雄性SD成年大鼠各20只,5周龄小雌、雄性SD鼠各20只,按照不同性别和鼠龄随机分组,每组10只,共8组。不同性别、年龄的4组大鼠每天以10mg/kgL-arg灌胃14d,另外4组给予生理盐水。待大鼠皮肤出现被毛潮湿、油腻、黄染等病理现象时,取血进行性激素检测,取皮肤组织进行组织学观察。结果L-arg灌胃7d左右,雄性成年大鼠组全部动物背部皮肤被毛潮湿、油腻、黄染,其血清中雄激素水平明显升高,组织学观察可见皮肤组织中皮脂腺异常丰富。雌性成年大鼠组用药时间至14d,8例动物有背部皮肤被毛潮湿、油腻、黄染发生,组织学改变同样是皮脂腺异常增生。有皮脂腺增生改变的动物血清中雄激素水平明显高于对照组,也高于未发生皮脂腺增生的动物。5周龄小鼠组雌雄动物需用药时间至14d全部动物才出现背部皮肤被毛潮湿、油腻、黄染,组织学改变也为皮脂腺异常增生,血清中雄激素水平明显高于对照组。结论L-arg可致大鼠正常皮肤皮脂腺异常增生,分泌增多,造成皮肤被毛潮湿、油腻、黄染发生,与L-arg及其体内产生的NO可使大鼠的雄激素分泌增多密切相关。
Objective To study the effect of L-arginine (L-arg) on the sebaceous gland of SD rat.Methods The SD rats of different sexes and ages were subjected to intragastric administration of L-arg at 10 mg·kg-1·d-1 (L-arg group) or saline (saline group) for 14 days.Blood samples were obtained from these rats for serum androgen determination and the pathological changes of the skin were observed.Results After L-arg treatment for 7 days,the dorsal hair of all the adult male rats appeared damp,greasy and yellow,while that of the adult and 5-week-old female rats looked normal.When the treatment was prolonged to 14 days,the same changes occurred in most of the adult female rats and all the 5-week-old female rats.The sebaceous glands of the abnormal skin showed hyperplasia in pathologic examination.The serum levels of androgen in rats with sebaceous gland hyperplasia were significantly higher than those in the control rats.Conclusion L-arg can promote sebaceous gland hyperplasia in rats in relation to increased androgen secretion induced by L-arg and NO.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第7期766-768,共3页
Journal of First Military Medical University
