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用半定量RT-PCR方法分析小麦TaMlo-A1c基因的表达 被引量：28

Expression Analysis of Wheat TaMlo-A1c Gene by Semi-QRT-PCR
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摘要 以小麦稳定表达的肌动蛋白基因(Actin)作为对照,利用半定量反转录聚合酶链式反应(Semi-QRT-PCR)技术,对与抗白粉病有关的小麦(TriticumaestivumL.)TaMlo-A1c基因的表达进行了研究。结果发现:TaMlo-A1c基因在小麦的叶、茎、根中均表达,穗中不表达,在白粉菌(Blumeriagraminis(DC.)E.O.Speerf.sp.triticiEm.Marchal,Bgt)诱导不同时间后小麦叶片中的表达稍微有所增强。研究表明,用半定量RT-PCR技术研究小麦基因表达,具有特异性高、操作简便和可靠性强的优点。 Expression analysis of wheat TaMlo-A1c gene,which was related to powdery mildew resistance in wheat,was carried out by semi-QRT-PCR,using wheat Actin gene as a control.The results showed that TaMlo-A1c gene was expressed in leaves,stem and root,no expression in spike.The transcribing of TaMlo-A1c in leaves were slightly enhanced by Blumeria graminis (DC.) E.O.Speer f.sp. tritici Em.Marchal ( Bgt ) infection.It was reliable,specific and easier to operate in researching.
作者 张丽娜 牛吉山 于玲
机构地区 西北师范大学生命科学学院 河南农业大学国家小麦工程技术研究中心
出处 《西北植物学报》 CAS CSCD 北大核心 2005年第7期1368-1371,共4页 Acta Botanica Boreali-Occidentalia Sinica
基金 河南省科技厅重点攻关项目(123014400) 河南省2002年度杰出青年科学基金资助项目(02120000800)
关键词 小麦 表达 TaMto—Alc基因 肌动蛋白基因 半定量反转录聚合酶链式反应 wheat expression TaMlo-A1c gene Actin gene Semi-QRT-PCR
分类号 Q786 [生物学—分子生物学]
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参考文献11

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二级参考文献64

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共引文献67

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引证文献28

二级引证文献102

西北植物学报
2005年 第7期

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