摘要
建立了一株人肾颗粒细胞癌细胞系（ＧＲＣ－１），在体外培养１１０代，历经１７个月。细胞生长和传代稳定，无其它细胞及微生物的污染。细胞为完全贴壁生长，群体倍增时间为１６小时，集落形成率为１４％，软琼脂培养平均集落为１７．５％，具有超二倍体核型，染色体众数为８２，流式细胞仪分析ＤＮＡ指数为１．８８，细胞在ＣｏｎＡ为３．１μｇ／ｍｌ时发生凝集反应，乳酸脱氢酶同功酶酶谱与正常细胞差异较大，为此细胞系所特有，有ＩＬ－６ｍＲＮＡ表达，培养上清中有较高ＩＬ－６生物活性表达。
A new cell line of human renal cell carcinoma (GRC-1)has been established and has been passaged 110 times in vitro for about one year and five months.The doubling time of this cell line was 16 hours, with a plating efficiency of 17.5 % in the double-layered soft agarose system.The typical model number of chromosome was 82,the DNA index being 1.88. The cell line expressed the biological activity of IL-6 and ILmRNA.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1995年第1期3-6,共4页
Chinese Journal of Urology
