摘要
从鼠的尿液中分离出肾钙素（ＮＣ），测分子量为１４６００，等电点（ｐＩ）为４．５～６．０，与正常人尿ＮＣ相似；应用维生素Ｋ拮抗剂香豆素可以减少鼠尿ＮＣ中的γ－羟基谷氨酸（Ｇｌａ）量，对其它氨基酸影响不大；用一水草酸钙（ＣＯＭ）种晶技术测量其对ＣＯＭ结晶生长，聚集抑制率也降低。结果提示：鼠肾产生的ＮＣ，其分子Ｇｌａ是ＮＣ合成后产生，通过维生素Ｋ参与谷氨酸（Ｇｌｕ）羧化形成，ＮＣ分子缺少Ｇｌａ可减低它对ＣＯＭ结晶生长，聚集抑制率。
Nephrocalcin(NC) was isolated from rat urine.The molecular weight was 14600 and the isoelectric point 4.5-6,being very similar to the urinary NC in normal human being.With the use of coumarol,a vitamin K-antagonist.it was able to reduce the r-carboxyglymin(Gla) in rat urinary NC while little effect was observed on other amino acids.The decrease of inhibitory effect on COM crystalization was also verified by the adoption of COM crystalization technique.The results denoted that the biosynthesis of Gla in NC occurs posttranslationally and involves vitamin K-dependent enzymatic carbozylation of specific glutamic acid in the polypeptide chain.Vitamin K-antagonist could reduce the turnover.It was concluded that Gla deficiency in NC plays an important role in the formation of urolithiasis.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1995年第10期583-585,共3页
Chinese Journal of Urology
