摘要
对８２例皮肤孢子丝菌病病理组织内的病原体做了观察，结果ＨＥ染色发现星状体２２例（２６．８％）、孢子３６例（４３．９％）。４５例ＰＡＳ染色发现星状体９例（２０％），孢子３８例（８４．４％），菌丝２例。５例多菌组织块透射电镜观察均见到孢子，并发现一个芽生孢子被中性粒细胞伪足钳住将被吞噬状。４例免疫荧光抗体染色均见到孢子。４５例组织液涂片革兰染色及ＰＡＳ染色发现孢子４０例（８８．８％）、星状体１例（２．２％）。５例扫描电镜观察见到各种形态孢子，１６例免疫荧光染色均见到孢子。真菌培养５２例中４７例（９０％）证实为申克孢子丝菌。孢子丝菌素皮试３８例均阳性。
In this paper, organisms in biopsy specimens of 82 cases of sporotrichosis were studied. The results showed that in the histopathological sections with HE stain asteroid bodies were observed in 22/82 (26.8%) sections, spores in 36/82 (43.9%) sections. of 45 sections with PAS stain asteroid bodies were found in 9 (20% ) sections,spores in 38(84. 4% ),and mycelia in 2. Spores Were found in all of 5 cases with multi-organisms under TEM, one spore was being wrapped by the pseudopodium of a neutrophil and going to be phagocytosed. Spores were found in all of 4 cases with fluorescent antibody staining. In smears exudates from lesion, spores were found in 40 cases (88. 8 % ) and asteroid bodies in 1 case (2.2 % ) with Gram and PAS stain in 45 cases. Spores with different shapes were found in 5 cases under SEM,and in all of 16 cases with fluorescent antibody stainig. The pathological tissues of 52 cases were cultured, and 47 cases (90%) were confirmed to be Sporothrix schenkii. Skin test of sporotrichin were performed in 38 cases,positive results were observed in all cases.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1995年第6期372-374,共3页
Chinese Journal of Dermatology
