摘要
应用逆转录－ＤＮＡ聚合酶链反应（ＲＴ－ＰＣＲ）及ＤＮＡ序列分析，对采自澳门地区的４３例抗－丙型肝炎病毒（ＨＣＶ）抗体阳性的慢性肝炎患者及５５例血液透析患者血清进行了ＨＣＶＲＮＡ检测及基因型分析。结果显示，１５例慢性肝炎患者中，８例为Ⅱ／１ｂ基因型，７例为６ａ型。在１１例ＨＣＶＲＮＡ阳性的血液透析患者中，１例为Ⅰ／１ａ型，５例为Ⅱ／１ｂ型，１例为６ａ型。这种基因型分布与在中国内地城市所得结果明显不同，提示丙型肝炎病毒在中国内地及相邻地区的广泛传播可能仅是近半个多世纪的事件。
Using reverse transcription-polymerase chain reaction based genotyping method and DNA sequencing, 43 sera from anti-HCV positive liver disease patients and 55 samples from hemodialysis in Macao were analyzed for HCV RNA and genotypes. The results show that out of 15 HCV RNA positive chronic hepatitis patients, 8 were genotype Ⅱ/1b 7 were type 6a. Among 11 HCV RNA positive hemodialysis patients, one was Ⅰ/1a, 5 cases were Ⅱ/1b, one was 6a. The pattern of genotypes distribution in Macao is apparently different from China's Mainland. It suggests that HCV spread widely in China's Mainland and the surrounding areas may occur recently.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1995年第3期202-205,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家"863"高技术研究发展计划及国家教育委员会资助
