摘要
应用逆转录聚合酶链反应(RT—PCR)检测11例血清抗HCV阳性的药瘾患者尸检肝组织和10例原发性肝细胞癌(PHC)患者手术切除肝癌组织中HCV基因(正链HCV RNA)和HCV复制中间体(负链HCV RNA)。结果8/11例肝组织和7/10例肝癌组织中正链HCV RNA阳性,其中各有6例负链HCV RNA亦阳性。HCV各基因区的检出率差别较大,5′NC区检出率最高、C区次之、NS区检出率较低。在同例肝(癌)组织中,若负链HCV RNA阳性,则其信号强度与正链基因无明显差别;但未发现肝癌组织中癌细胞分化程度与HCV的检出率有关。上述结果提示HCV感染与肝癌发生有密切关系,HCV的持续复制可能在癌肿形成中起作用。
11 cases of drug addicts with their serum anti-HCV positive and 10 cases of hepatocellular carcinoma were examined for HCV genes (plus strand RNA) and HCV middle replicated forms (minus strand RNA)in their liver tissues by reverse-transcription polymerase chain reaction(RT-PCR). The results showed plus HCV-RNA in the liver tissues of 8/11 cases and the cancer tissues of 7/1 0 cases were positive .minus strand HCV RNA of 6 cases in each group were positive. The positive rate of each region of HCV gene was much more different ,that of 5NC-region was the most. NS-region was the lowest. In the cancer tissues of a same case,if minus strand RNA of HCV was positive,its signal intension had no significant difference from that of HCV plus strand RNA. But the degree of differentiation of the cancer cells in the tissues of hepatocellular carcinoma was not found to be associated with the positive rate of HCV. The results above indicated that HCV infection were colsely associated with the incidence of hepatocellular carcinoma,and the constant replication of HCV might play a role in formating liver tumor.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1995年第2期116-119,共4页
Chinese Journal of Microbiology and Immunology
关键词
丙型肝炎病毒
复制中间体
聚合酶链反应
肝肿瘤
Hepatitis C virus
Minus strand RNA
Hepatocellular carcinoma
Polymerase chain reaction
