摘要
运用荧光原位杂交（ＦＩＳＨ）技术，检测了３１例慢性Ｂ淋巴细胞白血病（Ｂ－ＣＬＬ）中第１２号染色体三体（＋１２）畸变，７例（２２．６％）患者有＋１２，其中６例已经细胞遗传学检查发现有＋１２。在３例患者的外周血和淋巴结的淋巴细胞中，运用ＦＩＳＨ技术均检测到＋１２，而细胞遗传学检查仅在其淋巴结中发现有＋１２。表明ＦＩＳＨ在检测染色体畸变中比细胞遗传学方法敏感。＋１２是Ｂ－ＣＬＬ常见的特异性染色体畸变，淋巴结应做为Ｂ－ＣＬＬ进行染色体分析的首选材料。
y using fluorescence in situ hybridization (FISH),trisomy 12 in 31 patients with BCLL was assaved。Seven(22.6%)of 31 cases were found to have trisomv12,six of them had been detected bv chromosomeanalysis。 In three patients,Trisomv 12 was found notonly in lymph nodes but also in peripheral blood bvFISH, however,trisomy 12 was proved only in lymphnodes, but not in peripheral blood by chrornosomeanalysis. The results indicated that FISH was moresensitive than chromosome analvsis in the detection ofchromosome aberrations. Trisomy 12 was a commonchromosome aberration in B-CLL, and that lymphnode should be the first choice of specimen for chromo-some analysis in B-CLL.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第8期414-416,共3页
Chinese Journal of Hematology
