摘要
为了评估患者预后，用ＲＴ－ＰＣＲ方法研究了２７例急性髓细胞白血病（ＡＭＬ）思者骨髓细胞中ｍｄｒｌｍＲＮＡ表达，同时用３例正常人白细胞、敏感Ｋ５６２和耐药Ｋ５６２／ＶＣＲ细胞系作对照。结果发现所有复发与难治性ＡＭＬ患考ｍｄｒｌｍＲＮＡ均有较高水平表达（０．６９３±０．１０８），而初治组中１８例仅有５例ｍｄｒｌｍＲＮＡ表达（０．３０５±０．１１８），明显低于复发难治组（Ｐ＜０．００１）。正常人及Ｋ５６２细胞系无表达，而Ｋ５６２／ＶＣＲ有较高水平表达（０．８６）。结合患者病情分析，得出结论是ｍｄｒｌｍＲＮＡ表达可作为ＡＭＬ患者不良预后的预测指标之一。
xpression of mdrl mRNA in bone marrow cellsfrom 27 acute myeloid leukemia (AML) patients and 3normal subjects ,and in leukemic cell line K562 and itsresistant counterpart K562/VCR was assayed by re-verse transcriptase polymerase chain reaction. The re-sults showed that higher levels of mdrl mRNA weredetected in all relapsed and refractory patients (0. 693±0.108), 5 of 18 untreated patients (0. 305 ± 0. 1 18),and K562/VCR cells (0. 86), while in normal sub-jects and K562 cells the mRNA was undetectable. Itsuggested that expression of mdrl might be a predic-tive index for poor prognosis in AML patients.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第12期642-644,共3页
Chinese Journal of Hematology
基金
天津21世纪青年科学基金
