摘要
介绍采用BondElutC18小柱提取以反相高效液相色谱法同时测定尼弗地平、尼卡地平、尼群地平和尼莫地平4种钙离子拮抗剂的方法。应用Waters高效液相色谱仪,C18分析柱及乙腈/KH_2PO_4=46/54流动相,在1.5m1/min流速下分离上述4种药物。回收率分别为101%~109%,95%~101%,99%~104%和103%~108%,批内变异系数(CV)<7.4%,批间CV<8.7%。本方法特异性好,操作简便、迅速,适合于临床和药代动力学研究应用。
reversed phase HPLC method for simultaneous determination of nifedipine, nicadipine,ni-modipineand nitrendipine was developed.These analytes were extracted from 0.5ml serum using a solid phase Bond Elut Cl8 extract column and analyzed with Cl8 analytical column at 237nm.The minimum detection limit of nifedipine, nicardipine ,nimodipine and nitrendipine were 0.023μmol/L,0.029μmo1/L,0.022μmo1/L and 0.019μmo1/L respectively.The analytical recoveries were 95%~109%,and within day CV and day-to-day CV were<7.4%and 8.7%, Interferences from other drugs were tested and endogenous substrances in serum were not detected. It was found that the method was stable and suitable for both clinical routine and pharmacokinetics application.
关键词
尼弗地平
尼卡地平
拮抗剂
高效液相色谱
Nifedipine Nicardipine Nimodipine Nitrendipine Chromatography,high pressure liquid