摘要
本文报道一个经过改良的简单、快速、灵敏和准确的固相放射免疫分析法（ＳｏｌｉｄＰｈａｓｅＲａｄｉｏｉｍ－ｍｕｎｏａｓｓａｙ，ＳＰＲＩＡ），对其方法学、灵敏度和准确度进行了测定，并对其在检测法医物证几种生物检材血型物质应用方面进行了研究探讨。本方法批内变异系数为５．６％，批间变异系数为１０．１５％。新鲜血的稀释度达１：２５６０时，仍可用此法有效测出其中所含ＡＢＯ血型抗原。用该法分别检测人体微量血痕、毛囊组织和唾液斑的ＡＢＯ血型，获得满意结果。然后盲测５０例样本血型，以样本计数值大于８００次／ｍｉｎ判为阳性孔，小于８００次／ｍｉｎ判为阴性孔进行判型，结果全部正确。由于本法适用于各种微量生物材料血型物质的测定，因此在法医学领域内将会有较好的应用前景。
his paper reports a simple,rapid,sensitive and accurate solid phase radioimmunoassay (SPRIA)which has been improved.The research included the tests of its methodological pagroup substances in varied forensic biological materials.the coefficient variation of intraassay was 5.6%,and that of interassay was 10.15%.as to its applications to the forensic serology,the ABO blood groups of human bloodstains,hair follicular tissues and salivary stains had been tested and the results were satisfying.Later,50 unknown type blood samples had been blind tested.the judging level used to identify the positive and negative wells was 800 cpm,that meant,if the radioactive count of a well were over 800 cpm,it was determined as a positive well,if that of a well were below 800 cpm,it was negative well.as SPRIA is a method of microdetermination which can microtest the blood group antigens contained in varied forensic biological materials,it should has a good future of its applications to the forensic medicine fields.
出处
《中山医科大学学报》
CSCD
1995年第3期77-80,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
校青年基金
关键词
法医学
ABO血型
免疫学
放射免疫测定
forensic medicine
ABO blood-groups system/immunology
radioim-munoasssay/methods
