摘要
本文用ＰＣＲ，ＳｏｕｔｈｅｒｎＢｌｏｔ方法检测了３４只Ｇｏ代２６只转基因小鼠携带ＨＢＶＤＮＡ（阳性率为７６％），５１５只Ｇ１代转基因小鼠中２４８只组织中携带ＨＢＶＤＮＡ（阳性率为４８％），２５只Ｇ２代转基因小鼠中１８只组织中携带ＨＢＶＤＮＡ（阳性率为７２％）．对１０８只尼组织阳性小鼠血清检测，１０２只小鼠血清呈ＨＨＶＤＮＡ阳性。以上结果证明，ＨＢＶＤＮＡ基因已整合小鼠的染色体中。这些转基因小鼠对研究ＨＢＶ病毒的复制与表达，慢性肝炎以及肝癌发生的机理，治疗乙肝药物的筛选等具有十分重要的价值。
Hepatitis B virus (HBV) has an extremely narrow host range, and infects and replicates only in human and chimpanzee. The present experiment produced transgenic mice by microinjecting a partial tandem dupli-complete HBV genome adr type into fertilized ova of 34 mice,26 off springs (G0) were producers for HBV (76% HBV positive rate). The HBV genomes were transmitted to the next generation (G1) , and these G1 mice also produced HBV (248/515, 48% positive rate), 18/25 inbred G2 tail tissues carried HBV DNA (72% positive rate). Virus genome DNA was presentin sera of 102/108 G2 mice. Transgenic mouse containing HBV sequences is an useful tool for detailed study of replication and expression of HBV and for pathological study of hepatitis and hepato-cellular carcinoma.
出处
《肿瘤》
CAS
CSCD
北大核心
1995年第2期69-71,共3页
Tumor
