摘要
大鼠腹腔注射百草枯（５０ｍｇ／ｋｇ）后２４ｈ，血浆和肺组织匀浆内丙二醛（ＭＤＡ）含量、血浆和支气管肺泡灌洗液（ＢＡＬＦ）酸性磷酸酶（ＡＣＰ）和β－葡萄糖醛酸苷酶（β－Ｇ）活性均显著升高，肺湿／干重比、肺含血量和肺血管外水量（ＥＶＬＷ）也明显增加。组织学检查发现肺有充血、出血、局灶性肺不张和间质水肿。注射百草枯后５ｍｉｎ、６ｈ及１２ｈ腹腔内注射山莨菪碱（５０ｍｇ／ｋｇ），可明显减轻百草枯引起的肺损伤，抑制百草枯引起的ＭＤＡ含量和ＡＣＰ、β－Ｇ活性的升高。肺组织匀浆ＭＤＡ含量与ＢＡＬＦ内β－Ｇ活性之间，ＢＡＬＦ内β－Ｇ活性与ＥＴＬＷ之间均呈显著正相关。上述结果提示，脂质过氧化和溶酶体酶释放在百草枯致肺损伤中起重要作用；山莨菪碱可减轻百草枯引起的脂质过氧化反应和溶酶体酶释放，对百草枯性肺损伤有一定治疗作用。
Acute lung injury was induced in17 rats by a single intraperitoneal injec-tion of paraquat at the dosage of50mg/kg body weight. Twenty-four hoursafter paraquat injection, the malondialde- hyde(MDA)content in plasma and lunghomogenate,activity of acid phosphatase(ACP)and β-glucuronidase(β-G)inplasma and bronchoalveolar lavage fluid(BALF) were found to increase marked- ly The ratio of wet lung-to-body weight,lung blood volume and the amountof lung extravascular water(EVLW)alsoincreased significantly as compared withthe control values, Histological examin-ation of the lung revealed congestion,hemorthage, focal atelectasis and inters- titial edema in paraquat-treated anima ls. Treatment with anisodamine(50 mg/kgi.p.,given 5 min,6 h and 12 h afteradministration of paraquat)markedlyattenuated the paraquat-induced lunginjury as well as the increase in MDAcontent and the activity of ACP and β-G。Aclose correlation was shown be-tween MDA content in lung homogenateand β-G activity in BALF as well asbetween β-G activity in BALF and the amount of LEVW. These results sugge-ated that lipoperoxidation and lvsosomal enzyme release may be involved in the pathogenesis of acute lung injury caus-ed by paraquat,Anisodamine inhibits paraquat-induced lipoperoxidation and lysosomal cnzyme release, mitigates lung injury and may thus be used as a thera-peutic agent for paraquat poisoning.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1995年第4期338-343,共6页
Chinese Journal of Pathophysiology
