摘要
１６只成年杂种犬复制心肌缺血再灌模型。分为地奥心血康治疗组（ＤＡＸＸＫＧ）和生理盐水对照组（ＮＳＣＧ）。差速离心分离心肌细胞质膜，无机磷法测定心肌细胞膜Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶活性，荧光法测定心肌细胞膜与血清脂质过氧化物（ＬＰＯ）含量。结果表明：（１）ＤＡＸＸＫＧＮａ￣＋，Ｋ￣＋－ＡＴＰ酶活性明显高于ＮＳＣＧ（Ｐ＜０．０１）；（２）血清ＬＰＯ含量，随着再灌时间延长，ＮＳＣＧ呈上升趋势，ＤＡＸＸＫＧ略下降，再灌２４０ｍｉｎ两组比较差异显著（Ｐ＜０．０１）；心肌细胞膜ＬＰＯ含量，ＤＡＸＸＫＧ低于ＮＳＣＧ（Ｐ＜０．０５）；（３）心肌细胞膜Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶活性与ＬＰＯ含量呈明显负相关（ｒ＝－０．８３，Ｐ＜０．０１）。这些结果提示ＤＡＸＸＫ可通过抗脂质过氧化而实现其保护心肌细胞膜的效应。
Sixteen adult hybrid dogs induced myocardial reperfusion injury wererandomly divided into Di’Ao-Xin-Xue-Kang(DAXXK) treated group and normal saline(NS)control group. Myocardial cell membranes were seperated by different centrifugalspeed method.Na￣+,K￣+-ATPase activity was measured by inorganic phospliate determina-tion,LPO content of myocardial cell membranes and serum was measured according tofluorescence method. The results indicated that:Na￣+,K￣+-ATPase activity of DAXXKgroup was much higher than that of NS group ( P<0).②The more is the time ofreperfusion, the higher is the content of LPO in NS group. There was a reducing ten-dency in DAXXK group. At 240 min of reperfusion there was a significant differencebetween DAXXK group and NS group( P<0.01).LPO content of myocatdial cell mem-branes in DAXXK group was lower than that in NS group( P<0.05).③Myocardialcell membranes Na￣+,K￣+-ATPase activity was closely related to LPO content(r=-0.83,P<0.01)These results showed that the mechanism of the protecting effect of DAXXKon ischemia reperfused myocardium might be associated with the inhibition of cellularlipid peroxidation.
出处
《中国病理生理杂志》
CSCD
北大核心
1995年第4期409-412,共4页
Chinese Journal of Pathophysiology
