摘要
对来源于紫云英根瘤菌７６５３Ｒ总ＤＮＡ基因文库的５个互补结瘤菌株所分离到的重组质粒ｐＮＲ１０２，ｐＮＲ１０３，ｐＮＲ１０８，ｐＮＲ２０３和ｐＮＲ２１３，ＥｃｏＲⅠ酶切表明它们分别携带有一个４．６８，５．０１，５．０４，５．１０或９．３８ｋｂ的外源ＤＮＡ片段．选用１１种内切酶进行单、双酶切分析，建立了重组质粒外源片段的酶切图谱，５个质粒都具有１．７ｋｂ的ＥｃｏＲⅠ－ＢｇｌⅡ片段和１．９ｋｂ的ＥｃｏＲⅠ－ＳａｃⅠ片段．
Five recombinant plasmids which came from total DNA genes library of Rhizobium huakuii strain 7653R were isolated from the complementary nodulation strains R102,R103, R108, R203 and R213. They were named as PNR102, PNR103, pNr108, PNR203,PNR213 and contained respectively a 4. 68, 5. 01, 5. 04, 5. 10 or 9. 38 kb foreign fragmentwith EcoR I digestion. Their cleaving maps were made by analysis of single or double restriction endonucleases. The results showed that all recombinant plasmids contained a 1. 7kb EcoR Ⅰ-Bgl Ⅱ fragment and a 1. 9 kb EcoR Ⅰ-Sac Ⅰfragment.
出处
《武汉大学学报（自然科学版）》
CSCD
1995年第4期469-474,共6页
Journal of Wuhan University(Natural Science Edition)
基金
国家"攀登计划"资助
