摘要
用Ｆｕｒａ－２荧光测定法和ＲＩＡ，对２４例慢性肾功能衰竭（ＣＲＦ）维持血透患者（男１６例，女８例，年龄２６～７１岁，平均５０．９岁）血小板的胞浆游离钙浓度和血栓素Ｂ２（ＴＸＢ２）含量进行了测定。ＣＲＦ组透析前、后血小板胞浆游离钙浓度均高于正常对照组（Ｐ均＜０．００１），透析后低于透析前（Ｐ＜０．００２）；透前、后血小板ＴＸＢ２均低于正常（Ｐ分别小于０．００１和０．０５），透析后高于透析前（Ｐ＜０．００１）。血小板胞浆钙浓度变化与ＴＸＢ２生成不相关。Ｐ＜０．００１：与透析前比，ΔＰ＜０．０５，ΔΔＰ＜０．００２在相关分析上，Ｐｔ［Ｃａ２＋］ｉ与血小板ＴＸＢ２在正常对照组不相关（ｒ＝－０．２７，ｐ＜０．１），透析前、后也均不相关（ｒ＝０．３４和－０．２２，ｐ分别＜０．２和０．５）。讨论Ｃａ２＋是重要的细胞内信使，多种形式的细胞损伤或细胞处于病理生理状态下，常伴有胞浆钙水平的增高。血小板胞浆Ｃａ２＋在刺激信号的转导中发挥重要的作用，促使血小板的变形、聚集、释放，调节血小板的花生四烯酸代谢［３］。有关研究已证实，血小板［Ｃａ２＋］ｉ的变化能诱发内源性花生四烯酸代谢物的释放，参与ＴＸＡ２生成的调节；而ＴＸＡ２对［Ｃａ?
Platelet cytosolic free calcium (Pt[Ca2+]i) and thromboxane B2 (TXB2) contents in tweenty four uremic patients (male 16, female 18) on maintenance hemodialysis were determined by Fura-2 fluorometry and radioimmunoassay. Both pre-and post-dialysis Pt[Ca2-]i concentrations in uremic group were higher than that of the control group (P<0.001) and the post-dialytic content was lower than the pre-dialytic one. On the contrary ,TXB2 contents were decreased in both pre-and post-dialysis periods (P<0.001 and 0.05 ,respectively) and post-dialytic Pt[Ca2+]i concentration was not correlated with TXB2 content.
出处
《标记免疫分析与临床》
CAS
1995年第4期200-201,204,共3页
Labeled Immunoassays and Clinical Medicine
关键词
钙
血小板
血栓素B2
肾功能衰竭
血液透析
Calcium/platelet
Thromboxane B_2
Renal failure. chronic
Hemodialysis
