摘要
用３０ｋｅＶ六种剂量的氨、氢、氩三种离子注入四倍体陆地棉、二倍体中棉和草棉三个棉种的干种子，分析了Ｍ１胚根细胞的分裂指数以及核异常、微核和染色体畸变的类型及频率，比较了不同离子束细胞遗传效应的大小以及不同棉种对离子注入的敏感性。结果表明：三种离子注入均能明显地诱发根尖细胞核异常、微核和染色体畸变。氢离子诱发染色体畸变频率最高，其次为氩离子。。氢离子诱发染色体畸变中桥的比率较高，而氮、氩离子诱发的染色体畸变中落后染色体的比率高，陆地棉染色体对氢、氮离子注入的敏感性大于草棉和中棉。三种离子诱发陆地棉和草棉染色体畸变频率的剂量响应曲线不同于中棉.
Abstract Dry seeds of G.hirsutum,G.arboreum and G.
herbaceum were implanted respoctively by N+,H+and Ar+ with 3OkeV, in six doses. The
division index of root tip cells , the types andfrequencies of nuclear aberration ,micronucleu
and chromosomal aberration in M1 generation wereanalysed.The cytogenetic effects of
different ions beam and sensitivity of different cotton speciesto the three kinds of ion
implantafion were studied.The results showed that three kinds of ionimplantation could induce
significant nuclear aberration ,micronuclei and chromosomalaberration. Frequency of
chromosomal aberration induced by H+ was highest ,by Ar+ the next.The highist rate of
chromosomal bridges in chromosomal aberration induced by H+ was observed.The rate of
lagging chromosome in chromosomal aberration induced by N+, Ar+ was higher thanthat
induced by H+.The chromosomes of G. hirsutum to H+ and N+ implantation were moresensitive
than those of G.arboreum and C.herbaceum.The dose-effect curve of the
chromosomalaberration frequencies of root tip cells in C. hirsutum and C.herbaceum induced
by three kinds of ion differed from that in G.arboreum.
出处
《安徽农业大学学报》
CAS
CSCD
1995年第3期189-195,共7页
Journal of Anhui Agricultural University
基金
国家自然科学基金
