摘要
研究了影响牛血清白蛋白进入皂化二（２－乙基己基）磷酸酯（Ｐ＿（２０４））所形成的微乳液水池的因素，发现当萃取剂皂化度为８７％时对蛋白的溶解度最大；在所考察的范围内蛋白质的浓度一般无影响；对于盐效应来说，盐浓度增加对蛋白质进入微水池不利，影响程度按ＮａＣｌ，ＫＣＩ，ＣａＣｌ＿２的顺序依次增加；对于水相介质条件，当水相ｐＨ值为４到７时蛋白质较易进入微乳状液。利用ｐＨ和离子强度对蛋白质进入有机相的影响，可以通过调节ｐＨ和离子强度来实现萃取和反萃。
The injection method was used in this paper to investigate the solubilization of BSA in microemulsion of saponified bis(2-ethylhexyl)phosphate(P_(204)).When the saponification ratio of P_(204) is 87%, the volume fraction ( BSA) has the largest value. The concentration of BSA in aqueous phase has no obvious effect on BSA within the range of investigation. However, the increase of the concentration of salts decreases the value, and when the salt is CaCl_2 ,BSA has its largest decrease. The injection volume is relatively higher within the pH range of 4 to 7.These properties will be helpful in the separation and purification of BSA.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
1995年第6期690-694,共5页
Acta Scientiarum Naturalium Universitatis Pekinensis
基金
国家科委攀登计划项目
