摘要
建立了糖皮质激素受体(GR)的计数瓶培养测定法。用此法研究了10^(-8)、10^(-7)和10^(-6)mol/L地塞米松对人体肝癌细胞系(SMMC-7721)GR的影响,发现地塞米松使GR减少。去除地塞米松后24h,GR恢复到接近原来的水平。结果证明,糖皮质激素对GR呈现负调节。
The effects of dexamethasone(Dex)on the 3H-Dex specific binding were studied in the human heptatocellular carcinoma cell line(SMMC-7721) by using counting vial culture assay.Cells were cultivated in the counting vials, as th; grew well and the monolayer cells adhered to the wall of vials.The cells were cultivated in the mdium containing 10-3,10-7, and 10-6mol/L Dex for 6, 12, 24 and 48 h, and then the specific binding of 3H-Dex at 30nmol/L of the ligand was determined after elimination of the effect of the occupancy of GR.It was found that 3H-Dex specific binding was not changed within 6 h, but decreased to 70% at 12 h and maintained at this level from 12 to 48 h in the Dex pretreated cells.The specific binding was recovered approximately to the control level 24 h after removal of Dex.These results indicate that glucocorticoids may down-regulate glucocorticoid receptor in vitro.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1989年第1期26-28,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
糖皮质激素
GR
肝癌细胞系
glucocorticoids
glucocorticoid receptor
down-regulation of receptor human hepatocellular carcinoma cell
dexamethasone
