摘要
从两例鼻咽癌（ＮＰＣ）病人的活检组织切片中，用ＰＣＲ方法扩增出ＥＢ病毒潜伏感染膜蛋白（ＬＭＰ）基因的ＥｘｏｎⅠ、ＩｎｔｒｏｎⅠ、ＥｘｏｎⅡ和ＩｎｔｒｏｎⅡ共５００ｂｐ的片段，克隆入载体ｐＧＥＭ－３ｚｆ（＋）′，测定核苷酸序列，其中有一个样品所测序列段与台湾株相似，另一个样品则与Ｅ９５－８极为相似。由此可知，中国大陆南方的ＮＰＣ组织中ＥＢＶ－ＬＭＰ基因存在不同的变异。
DNA fragrnent containing Exon Ⅰ,Exon Ⅱ ExonⅡ IntionⅡof latentmembrane protein(LMP)gene of Epstein-Barr virus (EBV)obtained from two nasopharyngealcarcinoma biopsies(a and b ) by polyrnerase chain reaction (PCR)was cloned into pGEM-3zf (+).Sequencing and analysis revealed that biopsies a and b shared 94。4%and 99.6%homology respectively with B95-8 strai96.6% and 94.6% homology aspectively with theTaiwan Residents strain。So,it is suggestedthat there exist mutants differing in EBV-LMP insouthern China。
出处
《病毒学报》
CAS
CSCD
北大核心
1995年第1期10-14,共5页
Chinese Journal of Virology
关键词
潜伏感染膜蛋白
基因克隆
EB病毒
鼻咽肿瘤
Epstein-Barr virus(EBV) Latent membrane protein(LMP),Nasopharyngealcarcinoma,Polymerase chain reaction(PCR)Nucleotide sequence
