摘要
以桑籽经无菌继代培养的无性繁殖系叶片为材料，酶解分离得到原生质体；在Ｋ８ｐ液体培养基中，进行黑暗、静止、浅层培养，第４天细胞开始分裂，１０天以后形成细胞团；转移到弱光下培养，每隔１０天添加Ｋ８ｐ新鲜低渗培养液，经５－６周培养后形成大小不一的细胞团和小愈伤组织，转到含６－ＢＡ、ＮＡＡ的ＭＳＢ固体培养基上增殖培养，选取其中结构紧密、呈米黄色的愈伤组织在附加６－ＢＡ和ＮＡＡ的ＭＳＢ培养基上进行器管分化；在１／２ＭＳ附加ＩＢＡ的培养基上进行根的诱导，获得桑原生质体再生植株。
Mulberry protoplasts were isolated from leaves of young seedlings growing underaseptic condition.They were cultured in liquid K8p medium under still and darkcondition.The protoplasts started to divide since the 4th day.Cell colonies were formedafter 10 days.Then,they were put at a place where light is weak.Fresh K8p mediumwith low osmotic pressure was added to the cultures at an interval of 10 days.5-6 weekslater,cell colonies and calluses with different sizes were formed.Subsequently,they weretransferred onto proliferation medium containing 6-BA and NAA.Compact andyellowish calluses were transferred onto MSB medium supplemented with 6-BA andNAA for inducing organogenesis.Rooting was induced in 1/2MS medium containingIBA.
出处
《蚕业科学》
CAS
CSCD
1995年第3期154-157,共4页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金
