摘要
本文采用垂直板聚丙烯酰胺凝胶电泳方法，研究了安徽产游蛇科５种蛇的肌、肝、心、肾４种组织乳酸脱氢酶（ＬＤＨ）同工酶。结果表明：蛇类ＬＤＨ同工酶存在着明显的组织及种族的特异性，它们可以作为识别物种的生化指标。本文还利用各物种（ＯＴＵ）不同组织ＬＤＨ的Ａ、Ｂ亚基比值建立相似系数矩阵，进行聚类分析，初步探讨了种间的亲缘关系。
This paper reported LDH isozymes about four kinds of tissues of kidney,muscle,liver,and heart in five species of Colubridae:Opheodrys major(Guenther),Natrix tigrina lateralis(Berthold),Zaocys dhumnades(Cantor),Natrix annularis(Hallowell),Dinodon refozonatum(Cantor) by using polyacrylamide gel electrophoresis and optical density scanning.The results indicated that LDH isozyme of snake had tissue specificity and different LDH zymograms in each tissue of the animals,LDH zymograms may be used to identified species as one kind of index of biochemistry.In this paper,using A/B ratio of LDH isozymes of various tissue in five species established the matrix of euclidean distance coefficient and carried it analysis of fuzzy cluster.The reporter discussed preliminary the relation among five species in this paper.
