摘要
通过在ＲＰＨＰＬＣ固定相Ｃ１８表面键合氨基硅烷，引入氨基基团，将有机改进剂固定至硅胶键合相表面，克服了固定相表面硅羟基对含氮有机化合物的不可逆吸附。以最简单的ＣＨ３ＯＨ－Ｈ２Ｏ洗脱体系即能获得含氮有机物的最佳分离，从而可简化分离操作，提高柱寿命，降低分离成本。
By the reaction of aminopropyl triethoxy silane bonded silica phase with octodecanoyl chloride, a RP-HPLC packing with immobilized organic modifier for the separation of nitrogen-containing organic compounds was obtained. With this packing the separation of neutral organics is as good as the thaditional C18 bonded phase while for good separation of basic organics neither inorganic salts nor organic modifiers such as organic acids or bases are required in the mobile phase.
出处
《分析测试技术与仪器》
1995年第4期32-35,共4页
Analysis and Testing Technology and Instruments
关键词
有机改进剂
高效液相色谱
含氮有机物
分离
Silica-based stearamide packing, RP-HPLC, Column evaluation, Immobilization,Organic modifier.
