摘要
本文研究了新试剂安替比林基重氮氨基-2,4-二硝基苯(APDNBT)与汞的显色反应.在Tween-80存在下和pH=10.2～12.3的缓冲溶液中,APDNBT与汞生成黄色络合物,其络合比为Hg:APDNBT=1:2.建立了以445nm为参比波长,538nm为测量波长的双波长分光光度法,其表观摩尔吸光系数为2.1×10~5 L·mol^(-1)·cm^(-1),线性范围是0～15μg/25mL.成功地测定了天然水样及尿液中的汞,标准加入回收率为97%～104%,结果的相对标准偏差小于2.3%.本文研究了新试剂安替比林基重氮氨基-2,4-二硝基苯(APDNBT)与汞的显色反应.在Tween-80存在下和pH=10.2～12.3的缓冲溶液中,APDNBT与汞生成黄色络合物,其络合比为Hg:APDNBT=1:2.建立了以445nm为参比波长,538nm为测量波长的双波长分光光度法,其表观摩尔吸光系数为2.1×10~5L·mol^(-1)·cm^(-1),线性范围是0～15μg/25 mL.成功地测定了天然水样及尿液中的汞,标准加入回收率为97%～104%,结果的相对标准偏差小于2.3%.
A colour reaction on mercury with antipyrinyldiazoamino-2, 4-dinitrobenzene (APCDBT) and its optimum developing conditions were studied. In the presence of Tween-80, at pH range of 10. 2-12. 3, a 1 : 2(Hg : APDNBT) yellow complex was formed. A dual-wavelength spectrophotometric method for the determination of mercury with APDNBT was established, the reference and measurement wavelengths are 445 nm and 538 nm, respectively. The apparent molar absorptivity is 2. 10× 105 L · mol-1 · cm-1. Beer's law is obeyed in the range of 0-15 μgHg/25 mL. The method has been applied to the determination of mercury in natural water and urine with relative standard deviation of 2. 3% and recovery of 97% to 104%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1995年第5期579-581,共3页
Chinese Journal of Analytical Chemistry
关键词
尿
天然水
分光光度法
APDNBT
汞
Mercury, natural water, urine, antipyrinyldiazoamino-2,4-dinitrobenzene, spec-troptometry.
