摘要
提出了硫酸颜色反应用于利血平的荧光分析新方法.利血平与浓硫酸反应,生成强荧光物质,所得产物的荧光强度(λ_(ex)=400nm,λ_(em)=464nm)与利血平的浓度在0～0.6μg/mL范围内有良好的线性关系.检测限为0.2ng/mL.本法可直接用于尿液中利血平的定量分析,回收率为82.5%～84.2%.
A fluorimetric method for the determination of reserpine by color reaction of sul-furic acid is proposed. Reserpine reacts with sulfuric acid to form an intense fluorescent substance. The calibration curve is linear with the concentration of reserpine in the range of 0-0. 6 μg/mL. The limit of detection is 0. 20 ng/mL. The recovery of reserpine in urine is 82. 5%-84. 2%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1995年第9期1055-1058,共4页
Chinese Journal of Analytical Chemistry
基金
福建省自然科学基金
厦门大学育苗基金资助
关键词
利血平
硫酸颜色反应
荧光测定
尿液
Reserpine, color reaction, sulfuric acid, fluorimetric determination
